Template:HelpPage/Submission/Requirements
Submission Requirements
You've added your part to the Registry, but before you send a DNA sample to the Registry, here are the Registry's requirements for documenting parts and preparing part samples for submission. If you do not follow these requirements your submission will not be accepted.
If you have questions or issues concerning the requirements, contact hq (at) igem (dot) org. Please contact us early!
Documented
- Parts shipped to the Registry must be documented on the Registry.
- You must add your part to the Registry; it will then receive a part name (BBa_...)
- Your part's sequence must be entered into the Registry
- Annotate your part's sequence with features, add information on how your part works and how to use it best, and include characterization and measurement data
- Quality over quantity: sending a few well-documented parts is always better than sending several that are not
- For examples check out the collection of well documented parts.
Assembly compatible
- Parts must be BioBrick (RFC 10) or iGEM Type IIS compatible
- The Registry can test and maintain all parts in the same way
- Your part can be assembled to others.
- Users can easily move/assemble the part into another plasmid backbone
- Check if your part is BioBrick or Type IIS compatible by looking at the part's sequence and feature box.
- Remember, iGEM and the Registry is built on standard biological parts!
pSB1C3
- Samples must be in pSB1C3, the Registry's standard shipping backbone
- Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
- The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
- pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
- Users can easily move/assemble the part into another plasmid backbone
- You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.
Physical Shipment
- iGEM teams must follow these shipping guidelines for submitting samples of their parts
- Samples must be sent as dried miniprepped plasmid DNA, at least 250ng
- Submit in 96 well plates, sealed completely (adhesive foil/plastic), and covered with a protective lid.
- Submission shipments must be declared and labeled as DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)
- Samples must not be disguised or hidden to avoid customs
- Refer to Submission Kit help page for submission format details
- You can learn more about shipment safety on the [http://2019.igem.org/Safety/What_is_Safety#Safe_Shipment 2019"Safe Shipment" page]
Safety
- iGEM teams and labs must meet the standards set by the safety committee
- Teams should read through the 2020 iGEM Safety Hub to understand iGEM's Safety policies and rules.
- The Registry accepts parts from Risk Group 1 organisms.
- The Registry may accept parts from Risk Group 2 and 3 organisms that have received authorization from the Safety committee
- Please check your parts against the 2020 "White List" to see if any of them require more detailed documentation
Submission Form
- If your part meets the Registry's submission requirements, you're ready to send a DNA sample:
- you'll need to fill out an online submission form
- prepare the physical shipment using the submission kit (aliquot DNA into a 96well plate, dry down, cover, ship). Avoid leaky tubes!
- ship the DNA to iGEM HQ (a tracking number is required)
submission form instructions >>