Part:BBa_I739003

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes luxR (BBa_C0062) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. This LuxI/R system is the best characterized system of all [https://parts.igem.org/wiki/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is BBa_Q04121.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]