RBS

Part:BBa_K1992000

Designed by: Shilo Ohayon   Group: iGEM16_Technion_Israel   (2016-10-09)
Revision as of 21:16, 19 October 2016 by Shilo (Talk | contribs) (→‎Usage and biology)


Tar native RBS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and biology

This RBS was obtained from the genome of E.coli K12 and was located upstream to Tar encoding DNA sequence. The main use of this RBS is to examine its effect on the expression level of Tar chemoreceptor compared to the strongest RBS (BBa_B0034) according to the <a href="http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas" target="_blank">Warsaw 2010's measurement</a>.

Experiments and results

In order to compare the two RBSs, two expression plasmids constructed containing BBa_J23100 as promoter, the tested RBS, Tar receptor BBa_K777000 and terminator BBa_B0015. The two plasmids BBa_K1992004 and BBa_K1992005 were cloned to chemoreceptor free E.coli strain (UU1250) and tested by swarming assay as can be seen in figure 1. The larger radius of the native RBS suggested higher a sensitivity of the chemotaxis system that caused by a higher expression of Tar(1).

Fig1 (a) K1992004 - Tar expressed w RBS. (b) K1992005 - Tar expressed w the native RBS

Reference

1.SOURJIK, Victor; BERG, Howard C. Functional interactions between receptors in bacterial chemotaxis. Nature, 2004, 428.6981: 437-441.‏



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