Part:BBa_E0840

GFP generator

BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

Usage and Biology

• See BBa_E0040 for additional details.
• BBa_E0840 is often used to quantify the behavior of transcriptional control devices such as promoters.
• BBa_E0840 has a strong ribosome binding site.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 665
  

Improvements

Group: TU Delft 2016

Author: Lycka Kamoen and María Vázquez Vitali

Summary: Part BBa_E0840 does not contain any promoter, thus it can only be used if cloned downstream of one. It is also for this reason that this part (BBa_E0840) did not have any characterization. We have improved this BioBrick in 2 different ways: on the one hand, we provided 5 ready-to-go expression devices for green fluorescence (with promoters of different strengths), which can be used by future teams; on the other hand, this has allowed us to provide characterization for this uncharacterized GFP part. Using PCR primers, we added either of the constitutive promoters BBa_J23100, BBa_J23108, BBa_J23105, BBa_J23117 and BBa_J23113 to this part, to create five new biobrick devices. All of these biobricks were characterized by measuring their spectrum and growth cycle.

New parts: BBa_K1890020, BBa_K1890021, BBa_K1890022, BBa_K1890023, BBa_K1890024.

Characterization