Part:BBa_K1412801
Characterize efficiency of RBS with chemotaxis
BBa_K1412801: Plac-RBS(0.01)-CheZ-TT
This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by just change different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.
Usage
When we want to characterize the efficiency of RBS, we usually link the RBS with GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a CheZ gene, ending with a TT terminator(Plac-RBS(changeable)-CheZ-TT). Then transfer this gene circuit into E.coli (CheZ knocked out), and coat plates, culuture on semi-solid medium to measure the migration diameter of E.coli.
Relevant parts
Notes
Source
BBa_R0010 BBa_B0033 BBa_K629003 BBa_B0015
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protocol
Genetic link stage
1.As a skeleton, TT terminator link with target gene CheZ.
2.Then link the skeleton to target gene CheZ-TT.
3.Next, link RBS-CheZ-TT with promoter Plac.
Characterization stage
1.Transfer the part Plac-RBS-CheZ-TT into E.coli (CheZ knocked out), and coat plates, culture for hours to measure
the migration diameter of E.coli.
Reference
| None |