Part:BBa_K1412801

Characterize efficiency of RBS with chemotaxis

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BBa_K1412801: pLac-RBS(0.01)-cheZ-TT

This part consists of [http://en.wikipedia.org/wiki/Chemotaxis cheZ] gene which can express CheZ protein deciding E. coli whether tumble or swim straight.In this light, we can characterize the RBS or promoter efficiency by replacing different promoters or ribosome binding sites(RBS).Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.

Figure 1. The schematic diagram of how we characterize the activity of RBS

Usage

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When we want to characterize the efficiency of RBS, we usually connect the RBS with GFP, then measuring the fluorescence intensity of GFP. In our part, you need just connect RBS after a pLac promoter and before a cheZ gene, ending with double terminator(pLac-RBS(changeable)-cheZ-TT). Then transferred this gene circuit into E. coli (cheZ knocked out), and coated plates, culutured on semi-solid medium to measure the migration diameter of E. coli.

Relevant parts

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BBa_K1412000: pLac-RBS(1.0)-cheZ-TT CheZ generator under pLac promoter

BBa_K1412005: RBS(1.0)-cheZ-TT

BBa_K1412006: RBS(0.01)-cheZ-TT

BBa_K1412007: RBS(0.3)-cheZ-TT

BBa_K1412014: pTet-RBS(1.0)-cheZ-TT Characterize the efficiency of promoters with chemotaxis

BBa_K1412614: pBAD-RBS(1.0)-cheZ-TT Characterize the efficiency of promoters with chemotaxis

BBa_K1412829: Plac-RBS(0.3)-cheZ-TT Characterize efficiency of RBS with chemotaxis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

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More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]