Project
DarR
Part:BBa_K1045017:Design
Designed by: iGEM Team Göttingen 2013 Group: iGEM13_Goettingen (2013-09-20)
DarR reporter system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 806
Illegal NheI site found at 829
Illegal NheI site found at 909
Illegal NheI site found at 932
Illegal NotI site found at 718 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 860
Illegal AgeI site found at 168 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1630
Design Notes
DarR fused to the terminator were cut out from BBa_K1045016 and ligated in a pre-fixing composition into BBa_K1045015.
Source
This composite part was constructed using hybridization oligos for BBa_1045012 and BBa_1045011 or parts that were obtained either by amplification of plasmid DNA (BBa_1045009) or M. smegmatis chromosomal DNA (BBa_1045001). Part BBa_E0240 derived from the plasmid in the distribution kit 2013.
References
Lei Zhang, Weihui Li, and Zheng-Guo He (2013) âDarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatisâ, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085â3096