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Composite

Part:BBa_K1045015

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)

RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

This part consists of BBa_K1045014 (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator). This part also contains an inverse, strong RBS (BBa_K1045010) upstream of the inverse promoter. This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR (BBa_K1045001), if present.

Note: It might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
    Illegal NheI site found at 44
    Illegal NheI site found at 124
    Illegal NheI site found at 147
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845


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Parameters
n/aRBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240