Reporter
aeblue

Part:BBa_K1073020

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 13:56, 27 September 2013 by Laura S (Talk | contribs) (→‎Usage and Biology)

Constitutively expressed chromoprotein aeBlue

The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein aeBlue. This results in a constitutively high expression of aeBlue.

Usage and Biology

During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily.

Expressed chromoproteins.png AeBlue pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed (BBa_K1073022) and aeBlue.

Right: Cell pellet of E. coli XL1 expressing aeBlue.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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