Coding
amilCP

Part:BBa_K592009

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 14:01, 9 October 2012 by Erigu863 (Talk | contribs)

amilCP, blue chromoprotein

This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.

Usage and Biology

This part is useful as a reporter. In the pictures below it's built into BBa_K592015.

AmilCP amilGFP RFP.jpg On cultures BYR small.jpg Pellets BYR.jpg

iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red). Also see the green chromoprotein cjBlue BBa_K592011.

Getting this part

All chromoproteins displayed above should be available, but if the registry cannot send them, feel free to contact the Uppsala University team directly via erik.gullberg(at)imbim.uu.se, and we will send them to you.

References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
Parameters
abs588 nm
colorBlue