Part:BBa_K907002

Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS

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<Part Description>

This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for BBa_K907000(Mycobacteriophage Bxb1 integrase)/BBa_K907001(excisionase).
We designed this part to generate two different proteins one at a time.

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<Binary Signal Generation>
This part promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001. By doing so, this device can generate two different signals or binary signal. This binary signal can be further used in variety of modules or even in biological computing in the future.

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<Application>

Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation.

When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation is reversed. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step).

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The promoter reversed version is BBa_K907003.
This device is tested by GFP and mRFP attached construct. See BBa_K907004 and BBa_K907005.

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<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 75
  Illegal NheI site found at 98
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 157
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 22
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 42
  Illegal BsaI.rc site found at 125