Coding
Bxb1_Xis

Part:BBa_K907001

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)

Mycobacterium Phage Bxb1 gp47, DNA excisionase


KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes Bxb1 gp47, which is a DNA excisionase of Mycobacterium phage Bxb1.
The Bxb1 gp47 is a protein that directly binds to integrase-DNA complex. Without gp47, integrase-DNA complex recognize the "attL" and "attR" very weakly. The complex with gp47 strengthen the recognition and catalyzes DNA recombination reaction which is exactly reverse one done by integrase alone.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
Parameters
None