Part:BBa_K907002
Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
<Part Description>
This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for BBa_K907000(Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase).
<Binary Signal Generation>
This part promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001. By doing so, this device can generate two different signals or binary signal. This binary signal can be further used in variety of modules or even in biological computing in the future.
<Applications>
Initially, this device promotes the transcription and translation of downstream gene due to its promoter orientation. When Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase, recognizes and inverts the sequence flanking with attB and attP sequences, promoter orientation is inverted. Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step).
<Part Demonstration>
This device is tested by GFP and mRFP attached construct. See BBa_K907004.
<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 75
Illegal NheI site found at 98 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 157
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 22
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 42
Illegal BsaI.rc site found at 125
//direction/bidirectional
//promoter
//regulation/multiple
| None |