Part:BBa_K528020
Expression Adapter s03_65k
This part encodes expression adaptor designed to have strength 92,86% relative to B0034 and uniform expression level regardless of attached protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The part is in RFC25 standard. It consists of BBa_J23100 promoter followed by BBa_B0034 RBS, 5 nucleotide spacer and short peptide.
Spacer and peptide were designed using in silico evolution to provide uniform expression level regardless of following protein coding sequence.
Summary of design algorithm:
Measurements
Using RBSCalculator program we have determined that this part expression strength is 92,86% relative to BBa_B0034
All our measurement results are summarized in the table below:
| Part Name | Description | Expression level in thousands of absolute RBSDesigner units | Expression level as %B0034+YFP |
|---|---|---|---|
| BBa_K528015 | Expression Adapters s00_45k | 45 | 64,29% |
| BBa_K528016 | Expression Adapters s01_24k | 24 | 34,29% |
| BBa_K528017 | expression adapters s02_20k | 20 | 28,57% |
| BBa_K528018 | Expression Adapters s06_16k | 16 | 22,86% |
| BBa_K528019 | Expression Adapters s05_12k | 12 | 17,14% |
| BBa_K528020 | expression adapters s03_65k | 65 | 92,86% |
| BBa_K528021 | BBa_K528018+YFP measurement device | 16 | 22,86% |
| reference | BBa_J23102+B0034+YFP (100% reference construct) | 70 | 100,00% |
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/ExpressionAdaptors wiki page].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 8
Illegal NheI site found at 31 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |