Composite
Part:BBa_K525512:Design
Designed by: Jonas Aretz Group: iGEM11_Bielefeld-Germany (2011-09-14)
Polycistronic expression of BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 896
Illegal BamHI site found at 1284 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 421
Illegal AgeI site found at 383
Illegal AgeI site found at 1693 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 256
Illegal BsaI site found at 292
Illegal BsaI site found at 1503
Design Notes
- Polycistronic expression of BisdA and BisdB.
- Sequence of bisdA and bisdB was not entered correctly into the parts.igem. For further information see our notes in BBa_K123000:Experience and BBa_K123001:Experience
Source
- bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
- Anderson promoter BBa_J23110 and RBS BBa_B0034 from parts.igem
References
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].