Composite

Part:BBa_K525512:Design

Designed by: Jonas Aretz   Group: iGEM11_Bielefeld-Germany   (2011-09-14)

Polycistronic expression of BisdA and BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 896
    Illegal BamHI site found at 1284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 421
    Illegal AgeI site found at 383
    Illegal AgeI site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 256
    Illegal BsaI site found at 292
    Illegal BsaI site found at 1503


Design Notes

  • Polycistronic expression of BisdA and BisdB.
  • Sequence of bisdA and bisdB was not entered correctly into the parts.igem. For further information see our notes in BBa_K123000:Experience and BBa_K123001:Experience
  • bisdA and bisdB BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence


Source

  • Organism: bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
  • DNA (probably) synthesized and with optimated codon-usage for E. coli
  • All subparts can be found on one of the kit plates


References

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].