Status: 500 Content-type: text/html

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This BioBrick comprises a ligation of the registry parts for the promoter PcstA (BBa_K118011) and the RBS (BBa_B0034) to GFP (BBa_E0040) and a double terminator (BBa_B0015).

The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page here).

This part contains a GFP (green fluorescent protein) reporter in the functional transcriptional unit. As such, activity of the pCstA promoter can be assessed by the expression of GFP.

Usage and Biology

This construct was used by Imperial College's 2009 iGEM team for [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project using the following subparts: BBa_K118011, BBa_B0034, BBa_E0040 and BBa_B0015. It was ligated to BBa_J5526 to produce the final construct BBa_K200019.

Characterisation

Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured.

After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.

For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.

Sequence and Features Status: 500 Content-type: text/html

Software error:

Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250.
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.