Part:BBa_J45993:Design

Minimal stationary phase osmY promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'

Total Length: 49 bp

Annealing Length: 21 bp

GC Content: 38.1%

Melting Temperature: 49.5 degrees C

hairpin deltaG: -2.66 kcal/mol

self dimer deltaG: -99.97 kcal/mol

Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'

Total Length: 54 bp

Annealing Length: 25 bp

GC Content: 20.0%

Melting Temperature: 46.4 degrees C

hairpin deltaG: -2.86 kcal/mol

self dimer deltaG: -99.44 kcal/mol

heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol

Source

This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.

References

<biblio>

1. Yim pmid=8282684
2. Schellhorn pmid=9829938
3. Wise pmid=8631665
4. Funabashi pmid=11879714
5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.