Coding
THI3
Part:BBa_J45009
Designed by: Veena Venkatachalam Group: iGEM06_MIT (2006-06-07)
ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
- Very important note: None of the indicated base changes were made. Please consult design page to determine how we worked with this part.
This part catalyzes the second step in isoamyl alcohol biosynthesis. It converts alpha-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. 3-methylbutanal is then reduced via a alcohol dehydrogenase to isoamyl alcohol (the precursor to isoamyl acetate or banana scent).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3
Illegal EcoRI site found at 904
Illegal EcoRI site found at 1603
Illegal EcoRI site found at 1789
Illegal SpeI site found at 697 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/odor
s
s
ec_num | |
kegg | |
protein | THI3 |
swisspro |