Coding
THI3

Part:BBa_J45009

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)
Revision as of 20:27, 30 October 2006 by Rshetty (Talk | contribs)

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

  • Very important note: None of the indicated base changes were made. Please consult design page to determine how we worked with this part.

This part catalyzes the second step in isoamyl alcohol biosynthesis. It converts alpha-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. 3-methylbutanal is then reduced via a alcohol dehydrogenase to isoamyl alcohol (the precursor to isoamyl acetate or banana scent).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/odor
s
Parameters
ec_num
kegg
proteinTHI3
swisspro