Composite

Part:BBa_K1897019

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-11)
Revision as of 17:10, 17 October 2016 by Phuongnguyen (Talk | contribs)


p70-34-ALR-Terminator

The construct contains lldRO1-J23117-lldRO2 promoter designed by team ETH-Zurich 2015 (Part:BBa_K1847008) with a strong RBS (Part:BBa_B0034), followed by a coding sequence of Alanine Racemase (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal NheI site found at 500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 424
    Illegal BamHI site found at 1126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 542
    Illegal AgeI site found at 842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 299


[edit]
Categories
Parameters
None