Reporter
GFP genera

Part:BBa_E0840

Designed by: Jennifer Braff   Group: Antiquity   (2004-10-18)
Revision as of 10:42, 15 October 2016 by Lycka (Talk | contribs)

GFP generator

BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

Usage and Biology

  • See BBa_E0040 for additional details.
  • BBa_E0840 is often used to quantify the behavior of transcriptional control devices such as promoters.
  • BBa_E0840 has a strong ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 665

Improvements

Group: TU Delft 2016

Author: Lycka Kamoen and María Vázquez Vitali

Summary: We provided a ready-to-go expression device for green fluorescence, which can be used by future teams. Using PCR primers, we added the constitutive promoters BBa_J23100, BBa_J23108, BBa_J23105, BBa_J23117, BBa_J23113 to this part, to create five new biobrick devices. All of these biobricks were characterized by measuring their spectrum and and growth cycle.

New parts: BBa_K1890020, BBa_K1890021, BBa_K1890022, BBa_K1890023, BBa_K1890024.


[edit]
Categories
//classic/reporter/ret
s
Parameters
emissionGreen
excitation
tagNone