Regulatory
lacI+pL
Part:BBa_R0011
Designed by: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton Group: Antiquity (2003-01-31)
Promoter (lacI regulated, lambda pL hybrid)
Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:
- repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
- induced by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
Usage and Biology
Strong promoter. [jb, 5/24/04]
R0011 will be on in strains without lacI, off in strains that are lacIq (ie. Part:BBa_V1003) and medium in strains that are lacI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Intrinsic Noise Value: 0.0040 (compare with R0010: 0.0707; R0051: 0.0869). See [http://2015.igem.org/Team:William_and_Mary William_and_Mary iGEM 2015]
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
negative_regulators | 1 |
positive_regulators |