Cell
Part:BBa_V1003
Designed by: Group: Arkin Lab (2004-05-27)
D1210 (lacIq)
-- No description --
Usage and Biology
The following properties distinguish this strain from the other popular lab strain, [http://openwetware.org/wiki/E._coli_genotypes#HB101 HB101]:
- lacIq: Strain overproduces the lac repressor protein, lacI.
- lacY+: Strain deficient in lactose transport; deletion of lactose permease (M protein)
Features:
- Hybrid of E. coli K12 and E. coli B
- Host for pBR322 and many plasmids
- Sigma lists the deletion Δ(gpt,proA). Check this.
- Promega does not list F-, mcrB, or mrr
- Streptomycin resistant
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
genotype | HB101 lacIq, lacY+ |