Part:BBa_K1412614

Characterize the efficiency of promoter(BBa_K206000) with chemotaxis

BBa_K1412614: pBAD- RBS (1.0)-CheZ-TT

This part consists of a [http://en.wikipedia.org/wiki/Chemotaxis CheZ] gene which can express CheZ protein deciding E.coli whether tumbling or swimming straight. In this light, we can characterize the efficiency of promoter by just replacing different promoters before a CheZ gene. Then we can characterize the efficiency of promoter via measuring the migration distance positively associated with the expression strength of CheZ protein.

Usage

When we want to characterize the efficiency of promoter, we usually link the promoter with GFP, then characterize the efficiency of promoter by measuring the fluorescence intensity of GFP. In our part, you need just link RBS (1.0) after a pBAD promoter and before a CheZ gene, ended with a TT terminator (pBAD-RBS (1.0)-CheZ-TT). Then transfer this gene circuit into E.coli (CheZ knocked out), coat plates, and culture on semi-solid medium to measure the migration diameter of E.coli.