Translational_Unit

Part:BBa_K1465101:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-03)
Revision as of 20:32, 3 October 2014 by Tore (Talk | contribs) (→‎Design Notes)


Cytochrome c from Geobacter sulfurreducens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352
    Illegal SapI.rc site found at 433


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:
pSB1C3_pre_pccH (58 bp)

5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_pccH (43 bp)

5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'
  • Amplification of the cytochrome C (pccH) from Geobacter sulfurreducens KN400 using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:
pccH_fwd (55 bp)

5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'

pccH_rev (40 bp)

5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from Geobacter sulfurreducens containing also the RBS BBa_B0034.

Source

Gibson

References