Part:BBa_K1465101:Design

Cytochrome c from Geobacter sulfurreducens

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 352
  Illegal SapI.rc site found at 433
  

Design Notes

• Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:
  

pSB1C3_pre_pccH (58 bp)

5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_pccH (43 bp)

5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'

• Amplification of the cytochrome C (pccH) from Geobacter sulfurreducens KN400 using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:
  

pccH_fwd (55 bp)

5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'

pccH_rev (40 bp)

5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'

• [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from Geobacter sulfurreducens containing also the RBS BBa_B0034.
  

Source

• [http://www.ncbi.nlm.nih.gov/gene/?term=pcch%20geobacter%20sulfurreducens cytochrome c (pccH) from Geobacter sulfurreducens KN400]

References

• Dantas JM, Tomaz DM, Morgado L, Salgueiro CA (2013) Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens. [http://ac.els-cdn.com/S0014579313005139/1-s2.0-S0014579313005139-main.pdf?_tid=34116a64-4b3d-11e4-89d7-00000aacb35e&acdnat=1412368876_a3d9578217a3772893836e278ab068e6 FEBS Letters 587(16):2662-8]