Part:BBa_E0040:Experience

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User Reviews

UNIQd63d3f6225c9b2aa-partinfo-00000000-QINU

••• USTC_China iGEM13

In USTC_China iGEM13's project,we bulit the N-terminal TD1 modified GFP to make it is capable of transporting through skin barrier, then induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ultraviolet ultraviolet spectrometry. Students in Wen lab validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040 ). figure 1 SDS-PAGE of N-terminal TD1 modified GFP figure 2 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction figure 3 Our Transdermal test device figure 4 Results of transdermal test of N-terminal TD1 modified GFP and unmodified GFP Reference SUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4

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•• KAIST_iGEM_2012

Figure 1. E.coli strain MG1655 expressing BBa_E0040 under control of BBa_K907004 after overnight culture. 3mL culture with M9 media in 14 mL round bottom tube(Top left two) and centrifuged cells in eppendorf tube(Bottom left two). The expression of BBa_0040 is clearly observed with naked eye after overnight culture. BBa_E0040 was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-BBa_J23119 and RBS-BBa_B0034 in the Dual Phase Protein Generator(GFP default), BBa_K907004.

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••••• Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQd63d3f6225c9b2aa-partinfo-00000009-QINU

E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.

iGEM11_WHU_China

This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.

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