Part:BBa_K801060:Design

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1683
  Illegal SapI.rc site found at 107
  

Keywords: limonene synthase, limonene

Abbreviations:

Design Notes

Related BioBrick:

• BBa_K801061: (+)-Limonene synthase 1 coding region
• BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
• BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression

Quality control measures:

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick leads to the production of limonene. We investigated the toxicity of limonene and found out, that the growth of yeast strains was inhibited at concentrations of 1 mM. Limonene with a concentration of 100 mM even showed a lethal effect. Lower concentrations of limonene (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
BioBrick: BBa_I742111

Organism:

• Gene sequence derived from Citrus limon

References

Literature references:

• http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references:

• [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]