Generator

Part:BBa_K801063

Designed by: Lara Kuntz   Group: iGEM12_TU_Munich   (2012-09-21)

(+)-limonene synthase 1 expression cassette for yeast

This part can be used to express Citrus limon (+)-limonene synthase 1 in yeast. The expression is controlled by yeast TEF1 promoter and yeast TEF1 terminator.

Further information:

  • [http://www.ncbi.nlm.nih.gov/nucleotide/21435702 NCBI]
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
  • Origin of the enzyme: Citrus limon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211
    Illegal SapI.rc site found at 561


[edit]
Categories
//cds
//cds/biosynthesis
//chassis/eukaryote/yeast
Parameters
None