Coding
Part:BBa_K801060:Design
Designed by: Lara Kuntz Group: iGEM12_TU_Munich (2012-09-21)
(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1683
Illegal SapI.rc site found at 107
Protein data table for BioBrick BBa_K801060 automatically created by the BioBrick-AutoAnnotator version 1.0 | ||||||||||||||||||||||||||||||||||||||||||||||
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Nucleotide sequence in RFC 10: (underlined part encodes the protein) GTACACAATGCGTCGT ... TTCGAAAAATAA ORF from nucleotide position 8 to 1705 (excluding stop-codon) | ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
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Sequence features: (with their position in the amino acid sequence, see the list of supported features)
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Amino acid composition:
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Amino acid counting
| Biochemical parameters
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Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges | ||||||||||||||||||||||||||||||||||||||||||||||
Codon usage
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Alignments (obtained from PredictProtein.org)
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Predictions (obtained from PredictProtein.org) | ||||||||||||||||||||||||||||||||||||||||||||||
Subcellular Localization (reliability in brackets)
| Gene Ontology (reliability in brackets)
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Predicted features:
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Keywords:
limonene synthase, limonene
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
- Biobrick was sequenced
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- BioBrick encodes (+)-limonene synthase
- UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
Enzymatic activity:
- E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
Cytotoxicity:
- see toxicity assay
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- BioBrick: BBa_I742111
- Forward Primer: TUM12-LIMS1-fw-cons: 5'- GGATCCTCTAGAGTACACAATGCGTCGTTCAGCAAAC - 3'
- Reverse Primer: TUM12-LIMS1-rv: 5'- GGATCCACCGGTGCCTTTGGTGCCAGGAGA - 3'
Organism:
- Gene sequence derived from Citrus limon
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/12084056Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.]
Database references:
- [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]
- [http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]