Composite
Part:BBa_K784010
Designed by: Ilya Vainberg Slutskin Group: iGEM12_Technion (2012-09-15)
MCS+tac promoter+theophylline riboswitch+mCherry
This is a construct containing an MCS followed by a tac promoter followed by the theophylline riboswitch fused to mCherry. The construct was created using [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |