Regulatory

Part:BBa_K784008

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-15)

MCS+tac promoter

The tac promoter is a functional hybrid promoter, derived from the trp and lac promoters. The sequence here is preceded by an MCS as a by product of the restriction cloning steps from a pUC19 plasmid which contained the original sequence. The promoter was followed by the theophylline riboswitch and the complete construct was used for the [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] process with different proteins.
The promoter can be induced with [http://openwetware.org/wiki/IPTG IPTG].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 16
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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