Coding
amilCP

Part:BBa_K592009

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 17:02, 20 September 2011 by Erlu2699 (Talk | contribs)

amilCP, blue chromoprotein

This chromoprotein, amilCP, naturally exhibits strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible by removal of one illegal internal restriction site (EcoRI).

Usage and Biology

This part is useful as a reporter.

AmilCP amilGFP RFP.jpg On cultures BYR small.jpg Pellets BYR.jpg

The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
Parameters
abs588 nm
colorBlue