Part:BBa_K5205002

Amino acid 46-159 of OmpA, the extracellular fragment of outer membrane protein A from E. coli

The OmpA (outer membrane protein A) fragment from amino acids 46 to 159 is a segment of the protein that spans part of the extracellular domain in E. coli (Earhart, 2000).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

This partial OmpA is often fused with signal peptides such as Lpp or Non (BBa_K5205001, a signal-like sequence) to construct a cell surface display system, as seen in constructs like Lpp-OmpA (BBa_K1694010) (Earhart, 2000) and Non-OmpA BBa_K5205003 (Jeiranikhameneh et al., 2017).

References

Earhart, C. F. (2000). Use of an Lpp-OmpA fusion vehicle for bacterial surface display. Methods Enzymol, 326, 506-516. https://doi.org/10.1016/s0076-6879(00)26072-2

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0