Part:BBa_K5205003

Non-OmpA, cell membrane display system in Escherichia coli

This system helps display proteins on the cell membrane of E. coli. This part combines the Non signal-like sequence BBa_K5205001 with amino acids 46-159 of OmpA, encoding the extracellular fragment of the outer membrane protein A from E. coli (Earhart, 2000; Jeiranikhameneh et al., 2017).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Fusing this part with BBa_K5205000, which encodes OsMTI-1b, a metallothionein from rice, allows the fusion protein to be displayed on the E. coli cell surface and bind heavy metal ions in water.

References

Earhart, C. F. (2000). Use of an Lpp-OmpA fusion vehicle for bacterial surface display. Methods Enzymol, 326, 506-516. https://doi.org/10.1016/s0076-6879(00)26072-2

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0