Signalling

Part:BBa_K5205001

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-SDG   (2024-09-23)


Non, signal-like sequence

The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) BBa_K5205002 to form the Non-OmpA system BBa_K5205003. This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).


References

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0

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Parameters
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