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DNA
Part:BBa_K4438910
Designed by: Diya Raj N Group: iGEM22_IISER-Tirupati_India (2022-10-12)
crp_Target_5
crp_Target_5 (BBa_K4438910) is a single-stranded DNA having 87 nucleotides. The 5’ end has few bases complementary to crp_trigger_5_midtrigger_phi29(BBa_K4438909), the middle region has ssDNA sense T7 promoter sequence and the 5’ end has ssDNA sense broccoli sequence.
Usage and Biology
This part along with crp_trigger_1_P1_phi29(BBa_K4438901) and Aptamer_crp (BBa_K4438900) can be used to detect different concentrations of CRP [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.
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Categories
Parameters
None |