DNA

Part:BBa_K4438910

Designed by: Diya Raj N   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)

crp_Target_5

crp_Target_5 (BBa_K4438910) is a single-stranded DNA having 89 nucleotides. The 5’ end has few bases complementary to crp_trigger_5_midtrigger_phi29(BBa_K4438909), the middle region has ssDNA sense T7 promoter sequence and the 5’ end has ssDNA sense broccoli sequence.

Usage and Biology

This part along with crp_trigger_1_P1_phi29(BBa_K4438901) and Aptamer_crp (BBa_K4438900) can be used to detect different concentrations of CRP [1].

T--IISER-Tirupati India--hsa-crp-5.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.

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Parameters
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