Part:BBa_K4115039

J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015

J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
Function	Gene regulator
Use in	E. coli
RFC standard	RFC 10, RFC 10 compatible
Backbone	pBBR1
Submitted by	ShanghaiTech_China

This composite part is used to detect the response of LuxR (BBa_C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.

This part is togther used with LuxI (BBa_C0161), an AHL(3OC6 HSL here) sender.We can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals.

Proof of function

We encapsulated E. coli with this composite part and E. coli with LuxI into alginate gel beads. The E. coli with LuxI secreted the 3OC6 HSL signal molecule, and the E. coli with LuxR received the signal and regulate the expression of downstream sfGFP, making it express. So we can detect green fluorescence in the latter E. coli under the irradiation of ultraviolet light.

Figure 1: sfGFP is expressed under the regulation of LuxR between E. coli in a single gel bead. Two parallel groups are given and the above two are control groups.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 985