Coding

Part:BBa_K4115000

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-19)


Superfolder GFP (mammalian codon optimized)

This part is the coding sequence of superfolder green fluorescence protein(sfGFP). Its maximum excitation wavelength is 485nm. And its maximum emission wavelength is 510nm. sfGFP can fold correctly even when fused to some poorly folded proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

One of the usage examples in this project

Figure 1:Bacteria fluorescing during a carbon starvation promoter screening experiment. The strength of promoters can be compared indirectly by different brightness at unit OD600.
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Categories
Parameters
None