Plasmid_Backbone
pSB1AT3
Part:pSB1AT3
Designed by: Tom Knight Group: iGEM05_Registry_Example (2005-06-07)
High copy BioBrick assembly plasmid
pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AT3Usage and Biology
Tetracycline resistant up to 150 ug/ml.
Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.
- This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18903 (pSB1AT3F)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal NheI site found at 1270
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3431 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal BamHI site found at 1416
Illegal XhoI site found at 1035
Illegal XhoI site found at 2318 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3425
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3425
Plasmid lacks a suffix.
Illegal XbaI site found at 3440
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1442
Illegal NgoMIV site found at 1810
Illegal NgoMIV site found at 1970 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2464
[edit]
Categories
Parameters
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//plasmidbackbone/copynumber/high
chassis | |
copies | 100-300 |
insert | None |
mcs | BioBrick |
origin | pMB1 |
resistance | AT |