Plasmid_Backbone
pSB1AT3

Part:pSB1AT3:Design

Designed by: Tom Knight   Group: iGEM_Registry   (2005-06-07)


High copy BioBrick assembly plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal NheI site found at 1270
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal BamHI site found at 1416
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 2318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3425
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3425
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3440
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1442
    Illegal NgoMIV site found at 1810
    Illegal NgoMIV site found at 1970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2464


Design Notes

Source

pSB1A3 with added Tet resistance

References