Part:BBa_K2623013

Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC

Summary

As the first part of Kai circuit, it construct the the KaiC phosphorylation oscillation circuit.
The sequence encoding for the gene is preceded by a inducable promoter BBa_R0011, an RBS BBa_B0034, BBa_K2623001, BBa_K2623002, BBa_K2623003 and followed by a double terminator BBa_B0015 in pSB1C3 for periodic oscillation of KaiC phosphorylation.
The mechanisms by which cellular oscillators keep time and transmit temporal information are poorly understood. In cyanobacteria,the timekeeping aspect of the circadian oscillator, composed of the KaiA, KaiB, and KaiC proteins, involves a cyclic progression of phosphorylation states at Ser431 and Thr432 of KaiC.
Besides, with the aim to connect with BBa_K2623011 and fluorescent signals, we add a specific DNA sequence. The sequence encoding for the gene is preceded by the specific promoter BBa_K2623009, an RBS BBa_B0034, sfYFP BBa_K864100 and followed by a double terminator BBa_B0015 in pSB1C3 to be a part of the Kai circuit, which converts periodic oscillations of protein phosphorylation into periodic fluorescent signals by periodically activated RpaA.

Fig.1

Fig.2

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 627
  Illegal AgeI site found at 1442
  Illegal AgeI site found at 2153
  Illegal AgeI site found at 2162
  Illegal AgeI site found at 2168
  Illegal AgeI site found at 2492
  Illegal AgeI site found at 2519
  
• 1000
  COMPATIBLE WITH RFC[1000]