Regulatory

Part:BBa_K2623009

Designed by: Gezhi Xiao   Group: iGEM18_XMU-China   (2018-09-28)


pKaiBC promoter

Summary

As a component of the Kai circuit, pKaiBC promoter can be specific recognize and active by the phosphorylated RpaA，It participates in the construction of biological clock，as an important part of circadian rhythm-related protein expression in cyanobacteria.
The fragment is 89 bp.

pKaiBC in pathways

Fig.1 Simplified model of the central circadian oscillator and output activities.[1]
Fig.2 PKaiBC is one of the specific promoters that can be activated by RpaA-p.[2]

Reference

[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.
[2]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(7):2198-203.

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None