Part:BBa_J45009

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Usage and Biology

• THI3 is formerly known as KID1.
• 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).

• Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. Please consult design page to determine how we worked with this part.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 1000
  COMPATIBLE WITH RFC[1000]