Part:pSB1AT3

High copy BioBrick assembly plasmid

pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AT3

Plasmid map for pSB1AT3

Usage and Biology

Tetracycline resistant up to 150 ug/ml.

Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

• This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18903

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3425
  Illegal NheI site found at 1270
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3431
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3425
  Illegal BamHI site found at 1416
  Illegal XhoI site found at 1035
  Illegal XhoI site found at 2318
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3425
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3425
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3440
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 1442
  Illegal NgoMIV site found at 1810
  Illegal NgoMIV site found at 1970
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2464