DNA Submission
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Send a Part Sample to the Registry
You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it.
Mailing Address
Preparing your DNA Shipment
The Registry accepts miniprepped plasmid DNA (BioBrick RFC10 compatible part samples in pSB1C3, at least 10ul of 25ng/ul, or 250ng total if dried down) in single PCR tubes, 8-tube strips, or 96-well plates. Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.
See the pages below, for more in-depth information on requirements, submission form instructions, and how to prepare your physical shipment.
Things to keep in mind...
Documented
- Parts shipped to the Registry must be documented on the Registry.
- You must add your part to the Registry; it will then receive a part name (BBa_...)
- Your part's sequence must be entered into the Registry
- Annotate your part's sequence with features, add information on how your part works and how to use it best, and include characterization and measurement data
- Quality over quantity: sending a few well-documented parts is always better than sending several that are not
- For examples check out the collection of well documented parts.
Assembly compatible
- Parts must be BioBrick (RFC 10) or iGEM Type IIS compatible
- The Registry can test and maintain all parts in the same way
- Your part can be assembled to others.
- Users can easily move/assemble the part into another plasmid backbone
- Check if your part is BioBrick or Type IIS compatible by looking at the part's sequence and feature box.
- Remember, iGEM and the Registry is built on standard biological parts!
pSB1C3
- Samples must be in pSB1C3, the Registry's standard shipping backbone
- Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
- The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
- pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
- Users can easily move/assemble the part into another plasmid backbone
- You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.
DNA Submission
Requirements
Here's how part samples are sent to the Registry
Why pSB1C3?
Why do samples need to be shipped in pSB1C3?
Detailed Instructions
Instructions on how to fill out the Registry's submission form.
Submission Prep
Registry groups must follow iGEM's submission prep guidelines.