Part:BBa_K1031941:Experience

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User Reviews

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• TPR_China iGEM2020

This part is not such good in our own experiment. However, we changed the reporter from eGFP (BBa_K3599016) to sfGFP (BBa_K3599017), and the induction is rescued. The new part we made is BBa_K3599034. The fluorescence of the sensor induced by small molecules Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP. And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before. The fluorescence of the sensor induced by small molecules The fluorescence of the NahR sensor induced by small molecules The fluorescence of the sensor induced by PAN And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best. The fluorescence of the sensor induced by PAN

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