Coding

Part:BBa_K3599017

Designed by: Zijia Liu   Group: iGEM20_TPR_China   (2020-10-24)


superfolder GFP

GFP variant that folds robustly even when fused to poorly folded proteins. We used its sequence as a reporting gene to measure the intensity of the expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Reference

Pédelacq, J., Cabantous, S., Tran, T. et al. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol 24, 79–88 (2006). https://doi.org/10.1038/nbt1172

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