Part:BBa_K3599034
Pm-sfGFP
Overview
A reporter module of XylS sensor device. This module is an improvement version of BBa_K1031941 (Important!!!), which is designed by [http://2013.igem.org/Team:Peking Peking iGEM2013].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 38
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 188
Design & Experience
To characterize the property of the device itself, the original aromatic compond m-Toluic acid (mTa) was used as inducer. According to the experment results provided by [http://2013.igem.org/Team:Peking Peking iGEM2013], the mTa could cause the largest dynamic range and the most sensity of the Pm-eGFP sensor.
The fluorescence of the sensor induced by small molecules
Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP (BBa_K1031941). And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.
The fluorescence of the sensor induced by PAN
And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.
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